Journal: Toxics

Article Title: Machine Learning Model for Prediction of Development of Cancer Stem Cell Subpopulation in Tumurs Subjected to Polystyrene Nanoparticles

doi: 10.3390/toxics12050354

Figure Lengend Snippet: Summary of in vitro and in vivo studies using polystyrene particles.

Article Snippet: In vivo : BALB/c nude mice In vitro : human gastric cancer cell lines (AGS, MKN1, MKN45, NCI-N87, and KATOIII) [ ] , purchased from Cospheric (Somis, CA, USA) , polystyrene , 9.5–11.5 μm , In vivo : 1.72 × 10 4 particles/mL In vitro: 8.61 × 10 5 particles/mL , induced resistance to chemo- and monoclonal antibody-therapy.

Techniques: In Vitro, In Vivo, Polymer, Concentration Assay, Mouse Assay

Journal: Molecular imaging and biology

Article Title: HER3 PET Imaging Predicts Response to Pan Receptor Tyrosine Kinase Inhibition Therapy in Gastric Cancer

doi: 10.1007/s11307-022-01763-9

Figure Lengend Snippet: a Viability assessment of gastric cancer cells treated with 0.01–1 μmol/L of afatinib over 72 h demonstrates NCI-N87 as the most sensitive HER3-positive cell line, and SNU16 as a HER3-positive resistant cell line. b Western blot analysis of a panel of gastric cancer cells lines shows the baseline level of receptor tyrosine kinases and the AKT and MAPK nodes. Results are shown with three replicates

Article Snippet: Mouse models of human GCa were generated by subcutaneous implantation of 1 × 10 6 SNU16 or NCI-N87 cells (100 μL of 1:1 v:v, cell suspension in PBS: Matrigel) in the flank of athymic mice (male nu:nu, Charles River Laboratories, n = 64/tumor type).

Techniques: Western Blot

Journal: Molecular imaging and biology

Article Title: HER3 PET Imaging Predicts Response to Pan Receptor Tyrosine Kinase Inhibition Therapy in Gastric Cancer

doi: 10.1007/s11307-022-01763-9

Figure Lengend Snippet: a and b Treatment with 0.1 μmol/L afatinib results in inhibition of phosphorylated forms of RTKs and downstream AKT and MAPK in afatinib-sensitive NCI-N87 cells. In afatinib-resistant SNU16 cells, there is inhibition of phosphorylated forms of RTKs but sustained levels of activated AKT and MAPK. Results are shown with three replicates. ns, not statistically significant, * p < 0.05, ** p < 0.001

Article Snippet: Mouse models of human GCa were generated by subcutaneous implantation of 1 × 10 6 SNU16 or NCI-N87 cells (100 μL of 1:1 v:v, cell suspension in PBS: Matrigel) in the flank of athymic mice (male nu:nu, Charles River Laboratories, n = 64/tumor type).

Techniques: Inhibition

Journal: Molecular imaging and biology

Article Title: HER3 PET Imaging Predicts Response to Pan Receptor Tyrosine Kinase Inhibition Therapy in Gastric Cancer

doi: 10.1007/s11307-022-01763-9

Figure Lengend Snippet: Representative microscopic images of the cells on bright field and immunofluorescence staining with DAPI (blue color) and HER3 (red color) at 0–72 h of treatment with afatinib show overall decreased cell counts and increased HER3 signal in NCI-N87 cells; the %HER3/DAPI signal was significantly higher at 48–72 h of treatment with afatinib ( a and c ). In SNU16 cells, there is a continuous increase in the cell counts with decrease in the %HER3/DAPI signal over 72 h of treatment, although not statistically significant ( b and c ). (× 10 magnification) ( c ). ns, not significant, * p < 0.05

Article Snippet: Mouse models of human GCa were generated by subcutaneous implantation of 1 × 10 6 SNU16 or NCI-N87 cells (100 μL of 1:1 v:v, cell suspension in PBS: Matrigel) in the flank of athymic mice (male nu:nu, Charles River Laboratories, n = 64/tumor type).

Techniques: Immunofluorescence, Staining

Journal: Molecular imaging and biology

Article Title: HER3 PET Imaging Predicts Response to Pan Receptor Tyrosine Kinase Inhibition Therapy in Gastric Cancer

doi: 10.1007/s11307-022-01763-9

Figure Lengend Snippet: Coronal 68 Ga-HER3P1 PET and overlaid PET and T1-weighted MR (PET/MRI) images of tumor-bearing mice (male nu:nu mice, n = 12/ group) at baseline and 4 days after treatment with afatinib demonstrates significantly increased PET uptake in NCI-N87 tumors on day 4 ( a ), and no significant change in PET measures in SNU16 tumors between the two time points ( b ). K, kidney; ns, not significant, * p < 0.05 using paired t test, white arrow: tumor

Article Snippet: Mouse models of human GCa were generated by subcutaneous implantation of 1 × 10 6 SNU16 or NCI-N87 cells (100 μL of 1:1 v:v, cell suspension in PBS: Matrigel) in the flank of athymic mice (male nu:nu, Charles River Laboratories, n = 64/tumor type).

Techniques:

Journal: Molecular imaging and biology

Article Title: HER3 PET Imaging Predicts Response to Pan Receptor Tyrosine Kinase Inhibition Therapy in Gastric Cancer

doi: 10.1007/s11307-022-01763-9

Figure Lengend Snippet: Immunofluorescence staining of extracted tumor tissues ( n = 4 for each tumor type and each time point) for HER3 (red color) and DAPI (blue color) shows a significant increase in HER3 on day 4 and 21 post-afatinib treatment in NCI-N87 and unchanged to slightly decreased HER3 signal over time in SNU16 ( a and b ). Tumor growth curves demonstrate no significant change in the tumor volume in either group of sensitive or resistant tumors during the first 4 days of treatment with afatinib compared to their corresponding control group ( n = 16 per tumor and treatment type). Daily treatment with afatinib for 3 weeks results in a significantly decreased tumor volume in treated NCI-N87 group compared to control, and a continuously increased volume in SNU16 tumors ( c and d ). ns, not significant, * p < 0.05, (× 10 magnification)

Article Snippet: Mouse models of human GCa were generated by subcutaneous implantation of 1 × 10 6 SNU16 or NCI-N87 cells (100 μL of 1:1 v:v, cell suspension in PBS: Matrigel) in the flank of athymic mice (male nu:nu, Charles River Laboratories, n = 64/tumor type).

Techniques: Immunofluorescence, Staining, Control